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M9490104.TXT
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Document 0104
DOCN M9490104
TI Trans-activation of the 5' to 3' viral DNA strand transfer by
nucleocapsid protein during reverse transcription of HIV1 RNA.
DT 9411
AU Darlix JL; Vincent A; Gabus C; de Rocquigny H; Roques B; LaboRetro
INSERM, Ecole Normale Superieure de Lyon, France.
SO C R Acad Sci III. 1993 Aug;316(8):763-71. Unique Identifier : AIDSLINE
MED/94319978
AB Two DNA strand transfer reactions take place during reverse
transcription of the retroviral genome. The first transfer, that of the
minus-strand strong stop DNA from the 5' end of the viral RNA to the 3'
end, has been studied in vitro with two RNAs mimicking the 5' and 3'
regions of the HIV1 genome and with nucleocapsid protein, NCp7, and
reverse transcriptase. The results show that NCp7 strongly activates the
5' to 3' DNA strand transfer during reverse transcription while a basic
peptide resembling NCp7 is inactive. Activation of the first transfer by
several NCp7 derived peptides and the influence of the terminal
redundancies (R) present at the 5' and 3' ends of HIV1 RNA were also
examined. The first transfer is optimal in the presence of intact NCp7
and necessitates R on both the 5' and 3' RNAs. Sequencing of full length
viral DNA products reveals approximately 40% misincorporations at the
first nucleotide beyond the transfer point. If such base
misincorporations occur during proviral DNA synthesis with possible
homologous recombinations it may well contribute to the high level of
genetic variability of HIV.
DE Capsid/*METABOLISM DNA, Viral/GENETICS/*METABOLISM Gene Products,
gag/*METABOLISM *Genome, Viral HIV-1/ENZYMOLOGY/*GENETICS In Vitro
Reverse Transcriptase/METABOLISM Support, Non-U.S. Gov't
*Trans-Activation (Genetics) Transcription, Genetic JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).